萧玉涛课题组

萧玉涛课题组

　　Xiao Yutao Lab

　　

　　首席科学家/课题组长介绍：

　　萧玉涛，研究员，博士生导师，生态基因组研究中心副主任，中国农科院”青年英才”，深圳市高层次人才。长期从事昆虫分子生物学研究，在靶标害虫的抗药性机制、Bt抗性机制、寄主及环境适应性机制、重要昆虫基因组研究方面取得了一系列重要成果，以第一作者或通讯作者在Ecology Letters、PLoS Pathogens、PHILOS T R SOC B、JBC、Insect Molecular Biology等杂志发表论文12篇。主持国家自然科学基金、转基因生物新品种培育重大专项子课题等项目10余项，申请国家发明专利4项，获得授权1项，

　　Dr. Yutao Xiao, research fellow and doctoral supervisor, director of Centre of Ecological Genomics, is honored as "Outstanding Young Talent" of CAAS, "Shenzhen High-level Talent", and national "Ten Thousand Talent Program". He has been engaged in studies of insect molecular biology for a long time and made major progress in the analysis of pesticide resistance mechanism, Bt resistance mechanism, insect host and environmental adaptability，insect genomics. He has published 12 papers in SCI journals as (co-) first author or corresponding author, including Ecology Letters, PLoS Pathogens, PHILOS T R SOC B, JBC, and Insect Molecular Biology. Additionally, he has been awarded 10 projects supported by National Natural Science Foundation of China and National Major Project of Breeding for New Transgenic Organisms in China. One inventing patent has been authorized by the State Intellectual Property Office, and four items are being applied.

　　

　　研究成果：

　　1. Xiao Yutao, Liu Kaiyu, Zhang Dandan, Gong Lingling, He Fei, Soberón Mario, Bravo Alejandra, Tabashnik Bruce E, Wu Kongming. Resistance to Bacillus thuringiensis mediated by an ABC transporter mutation increases susceptibility to toxins from other bacteria in an invasive insect. PLoS Pathogens, 2016, 12(2): e1005450.

　　2. Xiao Yutao, Dai Qing, Hu Ruqin, Pacheco Sabino, Yang Yongbo, Liang Gemei, Soberon Mario, Bravo Alejandra, Liu Kaiyu, Wu Kongming. A single point mutation resulting in cadherin mislocalization underpins resistance against Bacillus thuringiensis toxin in cotton bollworm. Journal of Biological Chemistry, 2017, 292(7): 2933-2943.

　　3. Huang Xinzheng, Xiao Yutao, Köllner Tobias G, Jing Weixia, Kou Junfeng, Chen Jieyin, Liu Danfeng, Gu Shaohua, Wu Junxiang, Zhang Yongjun, Guo Yuyuan. The terpene synthase gene family in Gossypium hirsutum harbors a linalool synthase GhTPS12 implicated in direct defence responses against herbivores. Plant Cell and Environment, 2018, 41: 1-14.

　　4. Han Yongqiang, Wu Chao, Yang Lang, Zhang Deyong, Xiao Yutao. Resistance to Nilaparvata lugens in rice lines introgressed with the resistance genes Bph14 and Bph15 and related resistance types. PLoS ONE, 2018, 13(6): e0198630.

　　5. Jin Minghui, Xiao Yutao, Cheng Ying, Hu Jie, Xue Chaobin, Wu Kongming. Chromosomal deletions mediated by CRISPR/Cas9 in Helicoverpa armigera. Insect Science, 2018, 00: 1-8.

　　6. Xiao Yutao and Kongming Wu. Pecent progress on the interaction between insects and Bacillus thuringiensis crops. Philosophical Transactions B, 2019, 374.

　　7. Jin Minghui, Liao Chongyu, Swapan Chakrabarty, Zheng Weigang, Wu Kongming and Xiao Yutao. Transcriptional response of ATP-binding cassette (ABC) transporters to insecticides in the cotton bollworm, Helicoverpa armigera. Pesticide Biochemistry and Physiology, 2019, 154:46-59.

　　8. Jin Minghui, Liao Chongyu, Swapan Chakrabarty, Wu Kongming and Xiao Yutao. Comparative Proteomics of Peritrophic Matrix Provides an Insight into its Role in Cry1Ac Resistance of Cotton Bollworm Helicoverpa armigera. Toxins, 2019, 11: 92.

　　

　　团队研究方向:

　　面向国家农业重大需求，以重要农业害虫草地贪夜蛾、棉铃虫、地老虎等为研究对象，深入开展害虫的寄主及环境适应分子机制研究，力争为害虫绿色防控、食品安全、国家粮食安全做出重大贡献。主要包括以下三个研究方向：（1）靶标害虫抗药性、Bt抗性分子遗传机制及田间演化机制研究；（2）重要昆虫基因组及行为进化机制研究。

　　In order to meet the needs of national agriculture and focus on the green prevention and control of crop pests, the mechanism of plant-insect interaction was deeply studied in important agricultural pests, such as fall armyworm, cotton bollworm (Helicoverpa armigera) and black cutworm. There are two main research directions: (1) molecular genetic mechanism and field evolution mechanism of targeted pest insecticide resistance, Bt resistance; (2) genomics and behavior evolution mechanism of important insects.

　　

　　研究进展：

　　1.靶标害虫Bt抗性演化机制

　　The interaction between insects and Bt crops

　　全面梳理了Bt作物全球商业化种植以及在害虫防治方面的效果，并重点以棉铃虫和红铃虫为例归纳了Bt抗性三种不同的演化进程，从毒素激活相关酶的调控、Bt受体突变以及免疫系统调节三个方面详细总结了靶标害虫对Bt毒素抗性的机制，并结合生物学领域的新技术探讨了Bt抗性治理的新策略。

　　Reviewed the commercial cultivation of Bt crops in the world and their effects on target pest control. Taking the pink bollworm as an example, three different evolutional processes of Bt resistance are summarized. The mechanism of Bt resistance of target pests was summarized in detail from three aspects: regulation of related enzymes involved in the process of toxin activation, mutation or down-regulation of Bt receptor and regulation of immune system. A new strategy of Bt resistance management was discussed in combination with the new techniques in the field of biology in recent years, expecting to provide theoretical guidance for the long-term effective utilization of Bt crops and comprehensive control of agricultural pests.

　　2.小地老虎等基因组高质量组装行为进化机制研究

　　High-quality genome assembly of black cutworm

　　结合二代及PacBio测序数据，获得小地老虎基因组515 M，N50长度达到6.75 M，N90长度1.29 M，辅助Hi-C技术，成功将小地老虎基因组组装至染色体水平。课题组牵头发起“TOP1000昆虫基因组计划”，与国内外各单位合作逐步开展黄地老虎、红铃虫、大灰食蚜蝇等的基因组测序工作。

　　We successfully assembled 515 Mb genomic data of black cutworm, with N50 length of 6.75 Mb and N90 length of 1.29 Mb, through Illumina and PacBio sequencing. The genome was further assembled to chromosome level with Hi-C technology. The research team launched the "TOP1000 insect genome project", and cooperated with domestic and foreign units to carry out insect genome sequencing such as cotton bollworm, cotton pink bollworm, fall armyworm, Syrphidaes, etc.

　　3.昆虫基因组编辑研究

　　The platform of insect genome editing

　　建立了基于CRISPR/Cas9的鳞翅目昆虫基因编辑技术体系，采用注射双sgRNA的方法，实现了对棉铃虫目的基因大片段的编辑敲除，应用于HaABCC2以及HaCAD基因的编辑敲除试验，并成功获得相应的纯合品系，为昆虫基因大片段敲除提供了理论基础和技术参考。

　　A genetic editing system for Lepidoptera insects was established based on CRISPR/Cas9 technology. Using double sgRNA strategy, deletion of large fragments of the target gene of Helicoverpa armigera was realized. We successfully applied this technique in HaABCC2 and HaCAD genes and obtained corresponding homozygous lines. Providing theoretical basis and technical reference for large genome fragment knockout in insect.

 

萧玉涛课题组更新于2020年7月